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This project investigates the phyllosphere of Arabidopsis thaliana, beginning with a field study to collect samples from natural ecosystems in Tübingen, Germany over 6 years. DNA extracted from the leaves, followed by two distinct assays. The first assay, amplicon sequencing, used to characterize the leaf microbiome of bacteria and eukaryotes. The second assay, whole-genome sequencing, analyze the host genetic material.